IGA9e_ch05_labels - 061207

IGA9e_ch05_labels - 061207 - CHAPTER 5 The Genetics of...

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CHAPTER 5 The Genetics of Bacteria and Their Viruses Copyright 2008 © W H Freeman and Company
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CHAPTER OUTLINE 5.1 Working with microorganisms 5.2 Bacterial conjugation 5.3 Bacterial transformation 5.4 Bacteriophage genetics 5.5 Transduction 5.6 Physical maps and linkage maps compared
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Working with microorganisms
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Dividing bacterial cells Chapter 3 Opener
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The fruits of DNA technology, made possible by bacterial genetics Figure 5-1
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Bacteria exchange DNA by several processes Figure 5-2
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Bacterial colonies, each derived from a single cell Figure 5-3
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Distinguishing lac + and lac - by using a red dye Figure 5-4
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Table 5-1
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Model Organism Escherichia coli Model Organism E. Coli
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Bacterial conjugation
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Mixing bacterial genotypes produces rare recombinants Figure 5-5a
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Mixing bacterial genotypes produces rare recombinants Figure 5-5b
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No recombinants are produced without cell contact Figure 5-6
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Bacteria conjugate by using pili Figure 5-7
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F plasmids transfer during conjugation Figure 5-8a
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F plasmids transfer during conjugation Figure 5-8b
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Integration of the F plasmid creates an Hfr strain Figure 5-9
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Donor DNA is transferred as a single strand Figure 5-10
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Crossovers integrate parts of the transferred donor fragment Figure 5-11
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Tracking time of marker entry generates a chromosome map Figure 5-12a
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Tracking time of marker entry generates a chromosome map Figure 5-12b
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A single crossover inserts F at a specific locus, which then determines the order of gene transfer Figure 5-13
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The F integration site determines the order of gene transfer in HFRs Figure 5-14
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Two types of DNA transfer can take place during conjugation Figure 5-15
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A single crossover cannot produce a viable recombinant Figure 5-16
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Figure 5-17 The generation of various recombinants by crossing over in different regions
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Figure 5-18 Faulty outlooping produces F´, an F plasmid that contains chromosomal DNA
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IGA9e_ch05_labels - 061207 - CHAPTER 5 The Genetics of...

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