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Unformatted text preview: During eclipse, the virus must replicate its proteins, synthesize mRNA and/or go through genome replication. The virus then reassembles and goes through maturation. (Only enveloped viruses go through budding.) 3. You have a virus that transforms cells, but does not lyse cells. What specific assay can you use to quantitate this virus? (5 points) A typical assay for transforming virus is the focus-forming assay. However, you can also use changes in saturation density to measure multi-layer colony formation on plates (untransformed cells will form a monolayer), the ability of transformed cells to grow in low serum, or anchorage-independent growth in soft agar (assuming the normal cell type has anchorage-dependent growth). Hemagglutination assays would not work since some transforming viruses don’t make surface glycoproteins....
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This note was uploaded on 02/17/2010 for the course BIO 330 taught by Professor Dudley during the Spring '10 term at University of Texas at Austin.
- Spring '10