330qz208key - During eclipse the virus must replicate its proteins synthesize mRNA and/or go through genome replication The virus then reassembles

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Name__________KEY____________________ UT EID____________________________ BIO330 Quiz 2 February 7, 2008 Please read each question carefully before answering. 1. What is the difference between an infectious viral RNA and a positive polarity viral RNA? (5 points) An infectious viral RNA can be stripped of all proteins and introduced artificially into a susceptible cell, which then allows the entire infectious cycle and production of virions. A positive polarity RNA has the same strandedness as mRNA and can be translated into viral proteins. All infectious viral RNAs are positive polarity, but not all positive polarity viral RNAs are infectious. 2. Name at least two events that must occur during viral eclipse. (5 points) Eclipse is the period between viral entry/uncoating, when the virus loses its infectivity, and its subsequent replication into new infectious particles.
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Unformatted text preview: During eclipse, the virus must replicate its proteins, synthesize mRNA and/or go through genome replication. The virus then reassembles and goes through maturation. (Only enveloped viruses go through budding.) 3. You have a virus that transforms cells, but does not lyse cells. What specific assay can you use to quantitate this virus? (5 points) A typical assay for transforming virus is the focus-forming assay. However, you can also use changes in saturation density to measure multi-layer colony formation on plates (untransformed cells will form a monolayer), the ability of transformed cells to grow in low serum, or anchorage-independent growth in soft agar (assuming the normal cell type has anchorage-dependent growth). Hemagglutination assays would not work since some transforming viruses don’t make surface glycoproteins....
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This note was uploaded on 02/17/2010 for the course BIO 330 taught by Professor Dudley during the Spring '10 term at University of Texas at Austin.

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