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Unformatted text preview: transforming virus present can be visualized by a morphological change (focus formation) (also called a focus-forming assay), colony formation in soft agar (if the starting cells are anchorage dependent) or in low serum (transformed cells require less growth factors for growth). 3. Why do animal viruses need to use monocistronic mRNAs? (5 points) Animal viruses need to use the translational machinery of their hosts. Since mammalian ribosomes generally use only one AUG per mRNA to initiate translation, one mRNA generally leads to synthesis of one polypeptide. Thus, mammalian mRNAs and animal virus mRNAs are monocistronic (give one polypeptide per mRNA)....
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This note was uploaded on 02/17/2010 for the course BIO 330 taught by Professor Dudley during the Spring '10 term at University of Texas at Austin.
- Spring '10