330qz209key - transforming virus present can be visualized...

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Name______KEY______________ UT EID____________________________ BIO 330 February 12, 2009 Quiz 2 1. What is the first step in the infectious cycle of all viruses? What viral component mediates this step? (5 points) The first step in the infectious cycle is adsorption to the host cell. Adsorption is mediated by the viral anti-receptor, which can be an envelope glycoprotein or a capsid protein. 2. You have a virus that transforms cells, but does not give plaques. What specific assay can you use to quantitate this virus? (5 points) Transforming viruses can be quantitated similarly to plaque assays by plating virus dilutions onto monolayers of normal non-transformed cells. The quantity of
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Unformatted text preview: transforming virus present can be visualized by a morphological change (focus formation) (also called a focus-forming assay), colony formation in soft agar (if the starting cells are anchorage dependent) or in low serum (transformed cells require less growth factors for growth). 3. Why do animal viruses need to use monocistronic mRNAs? (5 points) Animal viruses need to use the translational machinery of their hosts. Since mammalian ribosomes generally use only one AUG per mRNA to initiate translation, one mRNA generally leads to synthesis of one polypeptide. Thus, mammalian mRNAs and animal virus mRNAs are monocistronic (give one polypeptide per mRNA)....
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This note was uploaded on 02/17/2010 for the course BIO 330 taught by Professor Dudley during the Spring '10 term at University of Texas at Austin.

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