16th revision notes

16th revision notes - Flagella contains a motor and protein...

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Flagella – contains a motor and protein filament and also a envelope that’s covers the filament (present in cells of some species) – allow the motility of the cell Fimbrae – the allow cell-to-cell attachment, protein structures Capsule – in pathogenic bacteria thy protect them from the defence system of the host, made of polysaccharides ( gelatinous glycocalyx) Frederick Griffith carried out ecperiements: Streptococcus pneumoniae (S and R form) S form contained capsule – pathogenic R form did not contain capsule – non-pathogenic Gram Stain – late 1800s 1. The sample is smeared onto glass slide 2. The slide is heated gently to allow cell attachment 3. Crystal violet is added ( stains the sample purple) 4. Iodine is added – forms a complex with crystal violet 5. Ethanol is added to decolourise - removes the complex from thin polysaccharide walls but not thick 6. Safranin is added a. Stains pink – gram negative – thin polysaccharide walls with layer on lipopolysaccharides on top
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This note was uploaded on 02/19/2010 for the course BIOL 10201 taught by Professor - during the Spring '10 term at Nottingham Trent.

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16th revision notes - Flagella contains a motor and protein...

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