bimm100ps4

bimm100ps4 - 11.What is the mechanism thought to produce...

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PS 4, 1 Problem Set #4 BIMM100 Winter 2010 – Short Answers 1. Define a gene. 2. What is the difference between a simple and complex transcription unit? 3. What does it mean when a gene is referred to as “solitary”? 4. Why is the sequence of duplicated genes less constrained? In other words, more likely to experience sequence drift? 5. What is a pseudogene? 6. What is the likely mechanism that results in duplicated genes or exons? 7. does this mean if an organism has a high gene density? 8. What percent of the human genome actually codes for proteins or functional RNAs? 9. In invertebrates and vertebrates, why are certain genes (such as genes coding for rRNA, tRNA and histones) present in multiple copies? Which of these are found in tandem arrays? 10. Name two general types of repetitive simple-sequence DNA (satellite DNA) discussed in class? What distinguishes one type from another?
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Unformatted text preview: 11.What is the mechanism thought to produce satellite DNA? 12. How can DNA be visualized after separating it on an agarose gel? 13. After separation on an agarose gel, are the large or small DNA fragments found near the wells on an agarose gel? 14.What does PCR stand for? Why is it such a powerful tool? 15. Name and describe the three steps in a cycle of PCR. 16. Why use a heat-stable DNA polymerase like Taq? 17. What happens if you use an annealing temperature that is above the melting temperature of your primers? What if you use an annealing temperature that is too low? 18. What must you consider when deciding how long your primers should be? 19. What happens if the cation concentration is too high in your PCR reaction? Too low? Explain. 20. How can PCR be used in DNA fingerprinting?...
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