Unformatted text preview: 11.What is the mechanism thought to produce satellite DNA? 12. How can DNA be visualized after separating it on an agarose gel? 13. After separation on an agarose gel, are the large or small DNA fragments found near the wells on an agarose gel? 14.What does PCR stand for? Why is it such a powerful tool? 15. Name and describe the three steps in a cycle of PCR. 16. Why use a heat-stable DNA polymerase like Taq? 17. What happens if you use an annealing temperature that is above the melting temperature of your primers? What if you use an annealing temperature that is too low? 18. What must you consider when deciding how long your primers should be? 19. What happens if the cation concentration is too high in your PCR reaction? Too low? Explain. 20. How can PCR be used in “DNA fingerprinting”?...
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This note was uploaded on 02/20/2010 for the course BIMM 100 taught by Professor Pasquinelli during the Spring '06 term at UCSD.
- Spring '06