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bimm100ps5 - 10 Name the enzymes/proteins encoded by each...

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PS 5, 1 Problem Set #5 BIMM100 Winter 2010 – Short Answers 1. Why are ddNTPs required for automated/cycle sequencing reactions but not for pyrosequencing? 2. In automated sequencing, which are fluorescently labeled, dNTPs or ddNTPs? 3. List the reagents/enzymes required for automated sequencing. 4. Of the two sequencing techniques discussed, which would be preferable to use if you wanted to sequence an entire genome? 5. In pyroseqeuncing, why would it be a bad idea to add all of the dNTPs at the same time? 6. Who was the first person to describe mobile DNA and in which organism? 7. Define transposable element. 8. Distinguish between “cut & paste” and “copy & paste” mechanisms. 9. Name the three families of TEs discussed in class.
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Unformatted text preview: 10. Name the enzymes/proteins encoded by each type of TE we discussed. 11. What feature is common to all mobile elements? 12. What is a difference between retroviruses and retrotransposons? 13. Distinguish between LINEs and SINEs. Which encodes the “tools” necessary to carry out the copy & paste mechanism? 14. Do non-LTR retrotransposons or viral-like retrotransposons target A/T-rich sequences in the genome? Why? 15. How can TEs influence the evolution of genomes? 16. What does it mean to say that eukaryotic cells have multiple genetic systems? 17. Of the organelles discussed in class, which uses the standard genetic code and which uses an alternate genetic code? 18. Where did organelles come from?...
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