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Homework 1 - Metabolic Biochemistry Fall 2009 Homework 1(35...

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Metabolic Biochemistry Name:____________ Fall 2009 Homework 1 (35 pts.) Section:___________ 1) (4 pts.) Does enzymatic catalysis affect the rate constant (k) for the reaction being catalyzed? Explain why or why not in detail. 2) (4 pts.) Does enzymatic catalysis affect the equilibrium constant for the reaction being catalyzed? Explain why or why not in detail. 3) (4 pts.) The following chemical reaction was set up and allowed to proceed to equilibrium: compound X compound Y + compouond Z Each compound was found to have the following concentration at equilibrium: compound X 8.2 x10 -6 M compound Y 1.7 x10 -2 M compound Z 1.7 x10 -2 M If the forward rate constant (k 1 ) for the reaction equals 128 sec -1 , calculate the value of the reverse rate constant (k -1 ). Show all of your work, and use the correct units on all constants.
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4) (5 pts.) The following data was collected from kinetic analysis performed on new enzyme: [S] ( μ mol/L) V 0 ( μ mol/L)/min 5 22 10 39 20 65 50 102 100 120 200 135 a. On graph paper, prepare a Lineweaver-Burke plot from the data and estimate V max and K M.
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