Week 3 Practice Problems - Practice Problems: 1. Effect of...

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Practice Problems : 1. Effect of enzymes on reactions Which of the listed effects would be brought about by any enzyme catalyzing the following simple reaction? k1 S P where K’ eq = [P] / [S] k-1 a) Decreased K’ eq a) Increased K’ eq a) Increased k 1 a) Increased k 2 a) Increased Δ G a) Decreased Δ G a) More negative Δ G’ ° 2. Applying Michaelis-Menten kinetics a) At what substrate concentration would an enzyme with a K M of 60 µ M operate at one quarter of its maximal rate? b) Determine the initial rates of the reaction (in terms of v max ) that would be obtained at the following substrate concentrations [S] : ½ K M , 2 K M , 10 K M c) Assume k cat = 20 min -1 . In an experiment, [S] = 20 mM, and the intitial velocity, v 0 was 480 nM min -1 . What was the total enzyme concentration [E t ] used in the experiment? d) In another experiment, [E t ] = 0.5 µ M, and the measured v 0 = 5 µ M min -1 . What was the substrate concentration [S] in the experiment? 3. Estimation of v max and K M by inspection (Lehninger Ch. 6, Qu. 11) Although graphical methods are available for accurate determination of the v max and K M of an enzyme-catalyzed reaction, sometimes these quantities can be readily estimated by inspecting values of v 0 at increasing [S]. Estimate the v max and K M of the enzyme-catalyzed reaction for which the following data were
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Week 3 Practice Problems - Practice Problems: 1. Effect of...

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