3-Practice Problems Key - 1. Effect of enzymes on reactions...

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1. Effect of enzymes on reactions Which of the listed effects would be brought about by any enzyme catalyzing the following simple reaction? a) Decreased K’ eq a) Increased K’ eq a) Increased k 1 a) Increased k 2 a) Increased Δ G a) Decreased Δ G a) More negative Δ G’ ° 2. Applying Michaelis-Menten kinetics a) At what substrate concentration would an enzyme with a K M of 60 µ M operate at one quarter of its maximal rate? insert into M-M: b) Determine the initial rates of the reaction (in terms of v max ) that would be obtained at the following substrate concentrations [S] : ½ K M , 2 K M , 10 K M Plugging this into M-M: Likewise: and Notice how at [S] = 10 K M , v 0 is already very close to v max . c) Assume k cat = 20 min -1 . In an experiment, [S] = 20 mM, and the intitial velocity, v 0 was 480 nM min -1 . What was the total enzyme concentration [E t ] used in the experiment? First, you need to realize that we’re at a substrate concentration [S], which is much higher than the K M . Thus, we’re at the special case where v 0 = v max . d) In another experiment, [E
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This note was uploaded on 02/21/2010 for the course MCB 102 taught by Professor Staff during the Spring '08 term at University of California, Berkeley.

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3-Practice Problems Key - 1. Effect of enzymes on reactions...

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