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2004_sp_1 - MICROBIAL GENETICS BID 366 — EXAM Fab 27...

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Unformatted text preview: MICROBIAL GENETICS BID 366 — EXAM] Fab. 27, 301214 Dr. Earhart Answer any 5 nftha first 6 questions; each is worth 2i} paints. Qufliun 7 is for Extra (mt-flit and is; warm 10 points. 1. Distinguish hehveen the fellewing: i. relaxed and supermiled DNA ii. stringent and relaxed plasmids iii. reversion and suppression iv. positive and negative regulafinn v. reeembinatinn and emnplemmtatinn i. What is a polar mutatinn‘? ii. Why am polar mutatiuns found ml}F in pmkaryotes‘? iii. Polar mutations can he fi-amashifi 0r nunstnse, but mt missrmsr: mutatinnfi. Explain. iv. Certain polar mutations can ha suppressed by mutations in the gene mending rho. Suggfit an explaatiun. Lu 1. Desm'he a procedure for isolating an anrpieiilin-reaistant mutant ofE. eoii. ii. Beaten—he a proeedure for isolating the origin of replication of" a plasmid. iii. Given: a plasmid library (pool, hank) ofE. eol'i DNA. and anE. colt I-lis' mutant. How would you isolate a plasmid earning the gene or genes that are defective in the His' mutant. Suggest possible explanations if your procedure fails to isolate the desired plasmid. 4. What are the tales, ifatty, of the fellowng entities in DNA replication? i. Gyrase ii. Okezeki fi'egmente iii. primese iv. heliease v. Pei: systems vi- DNA tigese 5. There are remarkable aimilaritias in the activities pfrclaxaaa proteins of self- tranamiaaabia plasmids and Lhai nf’REP proteins in railing-circle (RC) plasmid rcplicatiun- Explain. i. Define. mutation rate. ii. Dficfihe a procedure by Which a Imitatic-n rate can be asccrtajnad. {EXTRA CREDIT} ’3. Describe a plasmid expressinn vector. 1|What features must it have and what femures are desirablc {htlpfitl} to fulfill the fimction ofthis plasmid“? What might be a cunsequence to this plasmid if it cunmincld twn large direct repeats? ...
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