Molecular I pre-lab

Molecular I pre-lab - Molecular I Pre-lab 1. What is a...

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1. What is a plasmid? How is it different from genomic or chromosomal DNA? a. A plasmid is an extra chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently from the chromosomal DNA. It is circular and double-stranded and usually occurs naturally in bacteria but can be found in eukaryotic organisms. 2. What characterizes a cell as living? a. Responding to its environment, ability to reproduce, all living things are made up of cells, able to maintain a stable internal environment, all living things grow and develop, all living things obtain and use energy, as a species living things change over time 3. Is a plasmid a living cell? Can it grow or glow? a. Similar to viruses, plasmids are not considered a form of “life” as it is currently defined. Instead plasmids are considered transferable genetic elements, or “replicons”, capable of replication within a suitable host. A plasmid by itself cannot replicate itself. So, a plasmid does not grow. In the presence of UV light, the plasmid we will use in this lab will glow. 4. What is the name of the organism that grows on the agar plates and what type of organism is it? a. MM294, a strain of Escherichia coli which is a bacteria 5. What if E. coli had the plasmid pAMP in it? Which plates could it grow on and why? Use the words transformation, plasmid, antibiotic, phenotype, antibiotic-resistance and any other word that you feel is necessary in your explanation. a. The plasmid DNA can confer a different phenotype to the bacterial cell. The plasmid pAMP confers resistance to ampicillin to the cell. A cell that obtains this plasmid can survive in the presence of the antibiotic ampicillin, but cells that do not contain this plasmid will die because the antibiotic will kill the bacteria. The bacteria that has the pAMP inserted into it can grow everywhere but on the plate that contains kanamycin. 6.
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This note was uploaded on 02/21/2010 for the course BIOSC 0060 taught by Professor Barbarahertel during the Spring '07 term at Pittsburgh.

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Molecular I pre-lab - Molecular I Pre-lab 1. What is a...

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