Chem 243a - Extraction of Spinach

Chem 243a - Extraction of Spinach - 3A: Extraction of...

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3A: Extraction of Spinach Antonio Alarcon Chem 243a 9/17/07 I. Abstract In this lab, the structural components within spinach were isolated and identified (according to polarity) though TLC plate analysis. The lab concluded the following: xanthophyll proved to be the most polar while carotene proved to be the least polar (chlorophyll and pheophytin respectively followed between the two).
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II. Introduction In this lab, we were to use column chromatography to separate the different components in different fractions (by the use of solvents in increasing order of polarity: hexanes, 75/25 hex-acetone mix, and acetone) and then analyze the different fractions by TLC. This experiment demonstrated several techniques: separation, anhydrous drying, serial filtration, and TLC analysis (Padias, 29). Of the techniques presented in this lab, separation and serial filtration were of utmost importance. 75/25 mix was added to the spinach blend to aid the separation of the organic layer from the water and matter. This process (addition of 75/25 mix) was repeated as the organic matter did not want to separate. From this step, the spinach blend was centrifuged to separate, as mentioned above, the organic layer from the water and matter. The organic layer was then combined with anhydrous sodium sulfate to remove excess water without drying out the sample. This “dried” sample was then filtered to
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Chem 243a - Extraction of Spinach - 3A: Extraction of...

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