Lecture Notes 10-10-08 - – They can non covalently or...

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The sites where different classes of phosphlipases cleave phospholipids Phospholipases A1 and A2 cleave ester bonds and phospholipases C and D cleave phosphoester bonds
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Glycolipids Glycolipids are found exclusively in the outer leaflet of the cell membrane Glycolipids tend to self-associate and form lipid rafts Their roles in the cell are not well understood Thought to function in cell-recognition May have other functions
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Membrane Proteins Perform most of the specific functions of the membrane Account for approximately 50% of the membrane mass Are roughly 50 times the size of an individual phospholipid molecule Similar to membran lipids, the membrane proteins are often associated with oligosaccharide chains on the cell exterior
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Membrane proteins can associate with the lipids in various ways The transmembrane proteins can extend through and interact with the membrane (1-3)
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Unformatted text preview: – They can non covalently or covalently associate with only the cytosolic or external surface (4-6) – They can associate non covalently with other internal or external membrane proteins (7-8) • Only transmembrane proteins (1-3) can function directly on both sides of the bilayer, and can transport molecules across the membrane, for example Detergents Detergents • Detergents are commonly used to solubilize and purify membrane proteins • Detergents are amphipathic molecules, like phospholipids and transmembrane proteins, that surround the amphipathic membrane constituents and displace them from the membrane by disrupting their hydrophobic interactions • Detergents also denature, or unfold proteins • These features allow membrane proteins to be solubilized and analyzed by polyacrylamide-gel electrophoresis, where proteins are separated by molecular weight...
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