Lecture Notes 10-10-08 - – They can non covalently or...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The sites where different classes of phosphlipases cleave phospholipids Phospholipases A1 and A2 cleave ester bonds and phospholipases C and D cleave phosphoester bonds
Background image of page 2
Glycolipids Glycolipids are found exclusively in the outer leaflet of the cell membrane Glycolipids tend to self-associate and form lipid rafts Their roles in the cell are not well understood Thought to function in cell-recognition May have other functions
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Membrane Proteins Perform most of the specific functions of the membrane Account for approximately 50% of the membrane mass Are roughly 50 times the size of an individual phospholipid molecule Similar to membran lipids, the membrane proteins are often associated with oligosaccharide chains on the cell exterior
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Membrane proteins can associate with the lipids in various ways The transmembrane proteins can extend through and interact with the membrane (1-3)
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: – They can non covalently or covalently associate with only the cytosolic or external surface (4-6) – They can associate non covalently with other internal or external membrane proteins (7-8) • Only transmembrane proteins (1-3) can function directly on both sides of the bilayer, and can transport molecules across the membrane, for example Detergents Detergents • Detergents are commonly used to solubilize and purify membrane proteins • Detergents are amphipathic molecules, like phospholipids and transmembrane proteins, that surround the amphipathic membrane constituents and displace them from the membrane by disrupting their hydrophobic interactions • Detergents also denature, or unfold proteins • These features allow membrane proteins to be solubilized and analyzed by polyacrylamide-gel electrophoresis, where proteins are separated by molecular weight...
View Full Document

This note was uploaded on 02/22/2010 for the course BE 2400 taught by Professor Goldman during the Fall '08 term at Michigan Technological University.

Page1 / 8

Lecture Notes 10-10-08 - – They can non covalently or...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online