Lecture Notes 10-13-08 -...

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Detergents Detergents are commonly used to solubilize and  purify membrane proteins Detergents are amphipathic molecules, like  phospholipids and transmembrane proteins, that  surround the amphipathic membrane  constituents and displace them from the  membrane by disrupting their hydrophobic  interactions Detergents also denature, or unfold proteins These features allow membrane proteins to be  solubilized and analyzed by polyacrylamide-gel  electrophoresis, where proteins are separated  by molecular weight
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Movie 10.3
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Mouse-Human hybrid  cells provided evidence  for membrane protein  mobility Two different antibodies  were used to label  selected mouse and  human membrane  proteins The two sets of proteins  diffused and mixed over  the entire cell surface  within a half hour
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The diffusion rates of membrane proteins can be 
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This note was uploaded on 02/22/2010 for the course BE 2400 taught by Professor Goldman during the Fall '08 term at Michigan Technological University.

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Lecture Notes 10-13-08 -...

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