Lecture Notes 11-10-08 - Isolated 3T3 cell stained for...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Isolated 3T3 cell stained for F-actin (red), for DNA (blue), alpha -tubulin (green), a major component of the microtubule network. Images were recorded with a confocal microscope
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Green Fluorescent Protein (GFP)  can be used to tag individual  proteins in living cells and  organisms One of the simplest uses of GFP  is as a reporter molecule to  monitor gene expression, when  placed under the control of the  promoter belonging to the gene  of interest The GFP DNA-coding sequence  can be inserted at the beginning  or end of the gene for another  protein, yielding a chimeric  product consisting of that protein  with a GFP domain attached
Background image of page 2
SEM of hairs arising from a plant cell (A) Confocal microscopy of GFP tagged to an actin-binding protein
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Dynamics of GFP Tagging GFP is fused to a protein in the nucleus  and visualized with confocal microscopy  to reveal that the protein moves around  within the nucleus
Background image of page 4
Rapidly changing intracellular ion concentrations can be measured with  light-emitting indicators
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 14

Lecture Notes 11-10-08 - Isolated 3T3 cell stained for...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online