Lecture Notes 11-12-08-1 - ,thesourceof electrons

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Transmission Electron Microscope In the electron microscope, the source of  illumination is a filament that emits electrons The specimen is placed under a vacuum,  because particles in the air will scatter the  electrons The electrons pass through a tiny hole to form  an electron beam that is focused on the  specimen The specimen is stained with electron dense  material that scatters the electrons passing  through the specimen The dense regions of the specimen appear dark
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Specimen Preparation Because the specimen is exposed to a very high vacuum,  the specimen must be dry Tissues are usually preserved by fixation with  glutaraldehyde to cross link proteins and then with osmium  tetroxide to stabilize the lipid bilayers Water is often replaced with organic solvents Because electrons have limited penetrating power, the  fixed tissue must be cut into very thin sections (50 – 100 
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Lecture Notes 11-12-08-1 - ,thesourceof electrons

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