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Lecture Notes 12-1-08 - Splicing occurs positionsontheRNA...

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Splicing The nucleotide sequence signals where splicing  occurs Intron sequence removal involves three  positions on the RNA: The 5’ splice site The 3’ splice site The branch point in the intron sequence  In pre-mRNA splicing, each of these three sites  has a consensus nucleotide sequence However, there is enough variation in each  sequence to make it very difficult for scientists to  find all of these sites
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The Spliceosome Five short RNA molecules (U1, U2, U4,  U5, and U6) known as snRNAs (small  nuclear RNAs) recognize intron-exon  borders and participate in splicing Each is complexed with at least seven  protein subunits to form a snRNP (small  nuclear ribonucleoprotein) These snRNPs form the core of the  spliceosome, which performs pre-mRNA  splicing
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The spliceosome  recognizes the splicing signals on a pre-mRNA molecule  Brings the two ends of the intron together  Provides the enzymatic activity for the two reaction steps
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 Recognition of the three intron positions occur largely  through base-pairing between the snRNAs and the consensus  RNA sequences in the pre-mRNA substrate.
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