exercise 10 - Vivian Mai-Tran November 14, 2006 Lab 10...

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Vivian Mai-Tran November 14, 2006 Lab 10 Analysis 1. Gel Half #1 Traveled further (migration distance longer) More defined bands Big blotch for well #2 Spill over in well #3 M.W. and liver traveled the furthest (3.4cm) Gel Half #2 Didn’t travel as far as Gel Half #1 Less defined bands Big blotch on well #7 BSA and liver traveled furthest (3.2cm) Spill over in well #8 The electrotransfer of Gel Half #2 was not as successful as gel Half #1 because there were less bands present. The cause could have been from a shorter period of electrophoresis practiced or the technique used in both gels. 2. The Coomassie Blue stains everything including the tripe and liver proteins. The primary antibody detection system detects liver and tripe extracts’ serum albumin proteins. The secondary antibody detection system binds to primary antibody where the presence of alkaline phosphatase causes a faint blue-purple color to appear. 3. If two bands migrate to the same position in the gel, they are not necessarily composed of the same protein because a light, irregular shaped protein and a heavy spherical protein may travel the same distance yet be different. They might
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This note was uploaded on 02/22/2010 for the course BIO 50100 taught by Professor Brand during the Fall '09 term at University of Texas at Austin.

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exercise 10 - Vivian Mai-Tran November 14, 2006 Lab 10...

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