Unformatted text preview: their DNA genes. The X-gal in the agar is converted to a blue-colored compound by beta-galactosidase from the plasmids, resulting in a blue color that causes each transformed colony to appear blue. IPTG permits synthesis of beta-galactosidase with the removal of the repressor beta-galactosidase gene of Lac Z. 5. The white colonies because they contain the foreign new gene which interferes with the genetic sequence that makes beta-galactosidase enzyme thus it did not turn blue....
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This note was uploaded on 02/22/2010 for the course BIO 50100 taught by Professor Brand during the Fall '09 term at University of Texas.
- Fall '09