key 8 - KEY: Bio 205L EXERCISE 8 Prep Sheet for LABORATORY...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
KEY: Bio 205L EXERCISE 8 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory: If the same technique is used in multiple sections of a lab, it does not need to be listed multiple times. ( For the lab practical exam, be sure you know how to properly perform each technique). Section 8.1: Preparation of Crude Protein Extracts from Bovine Liver and Tripe Tissue. a. Using liquid N 2 to keep tissue frozen while grinding it to a fine powder in a mortar and pestle. b. Quickly transferring dry powdered tissue to an ice cold Dounce Homogenizer using a chilled spatula. c. Proper procedure for grinding powdered tissue and keeping it cold in a Dounce homogenizer. Section 8.4: Preparation of Standard Protein Solutions by the Parallel Dilution Method a. Proper use and operation of the spectrophotometer. b. Preparation of a series of protein solutions of known concentration from a stock solution by making parallel dilutions. c. Proper use and application of the formula C1 X V1 = C2 X V2 Section 8.5: Quantification of Total Protein Using the Bradford Assay a. Proper use and operation of the spectrophotometer. b.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

key 8 - KEY: Bio 205L EXERCISE 8 Prep Sheet for LABORATORY...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online