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dec 6 dna libraries - A p p r o x im a te M a x im u m L e...

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1 Making DNA Libraries Size of fragments Size of genome Number of fragments needed DNA VS. RNA (cDNA) library Screening the library Vector Type Cloned DNA (kb) Plasmid 20 Phage 25 Cosmid 45 P1 phage 100 BAC (bacterial artificial chromosome) 300 YAC (yeast artificial chromosome) 1000 Approximate Maximum Length of DNA That Can Be Cloned in Vectors 4 kb 333 bases 9.5 kDa intron intron Cloning Human Insulin
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2 17.9 Figure 17.9 4 kb 333 bases 9.5 kDa Size of human genome ~3.2 X 10 9 bp 3.2 X 10 6 kbp Average size of insert ~10 kb
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