dec 8 pcr - Polymerase Chain Reaction 1 2/8/0 6 Major ideas...

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Polymerase Chain Reaction 12/8/06 Major ideas and questions: 1. Fragments of DNA can be amplified by a technique called polymerase chain reaction (PCR). 2. PCR is extremely sensitive and can amplify sequences from a single cell. 3. PCR depends on heat-resistant bacterial DNA polymerases. 4. PCR can be designed to clone genes or to detect specific sequences in a genome. Reading: Attached reading from: Molecular Biology of the Cell , by Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson Chapter 7. Recombinant DNA Technology Website: http://www.dnalc.org/ddnalc/resources/animations.html http://www.dnalc.org/ddnalc/redirect.html?/shockwave/pcranwhole.html Look at the polymerase chain reaction animation at this site.
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Selected DNA Segments Can Be Cloned in a Test Tube by a Polymerase Chain Reaction The availability of purified DNA polymerases and chemically synthesized DNA oligonucleotides has made it possible to clone specific DNA sequences rapidly without the need for a living cell. The technique, called the polymerase chain
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This note was uploaded on 02/22/2010 for the course BIO 51980 taught by Professor Buskirk during the Fall '06 term at University of Texas.

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dec 8 pcr - Polymerase Chain Reaction 1 2/8/0 6 Major ideas...

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