oct 11 - Recombination and Gene Mapping 10/11/06 and...

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Recombination and Gene Mapping 10/11/06 and 10/13/06 Major ideas and learning goals: 1. Recombination contributes to acquisition of new genes by prokaryotic organisms and to genetic diversity in eukaryotes. 2. You should be able to show how this process occurs at the molecular level. 3. You should be able to use data on recombination frequencies to determine linkage of genes. Why would you want to know about linkage of genes? Textbook questions Chapter 5 has a number of problems at the end of the chapter that will give you practice in answering the questions posed above and will allow you to practice using the information. A suggested set of problems to work on is: 8, 9, 11, 13, 14, 16, 18, 20, 24, 34
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1 Double stranded break formation Resection 5 end on each side of break are degraded to produce two 3 single stranded tails First strand invasion RecA binds 3 tail and double helix allowing invasion and migration Formation of Holliday junctions New DNA synthesis forms two X structures -
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oct 11 - Recombination and Gene Mapping 10/11/06 and...

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