study lab practical

study lab practical - BIO 135 Lab Practical Review...

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BIO 135 Lab Practical Review Lab1 -Know p. 24-27 (Basic Lab and Safety Procedures) -How to dispose of the following materials (these are only a few examples) 10 mL of acid - neutralize with water and pour down the drain with lots of water Agar plate containing E.coli- Tape and put in orange biohazard bag Test Tube containing B.subtilus – Put bleach in test tube, allow to sit, pour down drain with lots of water Kim wipe from pH meter- trash can ** TIP: A question may be asked that seems general but BEWARE…if something general such as a glove or Kim Wipe contains something biological (cells, bacteria, etc.), these items need to be disposed of in biohazard bags NOT the trash. Be aware of what is on the substance you are disposing of…makes a in difference in whether it goes in the trash or biohazard.** Lab2 Taring and Using a Beamed Balance - Maximum capacity 500g - Know how to tare (see page 37) Taring and Using an Electronic Balance - Maximum capacity 300 g - Know how to tare (page 37) –Taring the electronic balance includes using the 200 g weight!! Know how to use the Vernier Caliper – you will probably be asked to measure an object (page 39) Lab3 Lab 3 is a difficult lab to utilize for a lab practical…this does not mean don’t study it! Glance over the Drosophilia stuff and make sure you know how to do a t-test and Chi- squared. Dr. Iten is notorious for finding ways to incorporate a topic that may be unlikely! Lab4 Be able to perform a serial dilution…we have been doing these all semester! Ex. Make a 7-fold serial dilution with the final volume containing 21 mL in the last beaker (using 4 beakers) see page 102-103 for other examples 21mL/7 = 3 mL stock solution taken from stock bottle and put into first beaker 1 part chemical and 6 parts diluent = 7 parts total Therefore, add in 6 X 3mL = 18mL of diluent (usually distilled water)
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*** TIP: Before transferring any liquid from your first beaker to your second, make sure you thoroughly mix the first beaker! Also, use a clean pipette every time you transfer liquid.*** Calibrating and Using a Pipetman - With the plunger at the 1 st stop, immerse in water and allow plunger to return to original position. Note: Do not let it snap up – point reduction by the TAs - When dispensing, push plunger to 1 st stop then further eject fluid by pushing plunger to 2 nd stop. ** NOTE- The TA grading you will watch that you go to the correct stops**
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study lab practical - BIO 135 Lab Practical Review...

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