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Biol223_Smith_08_2004-2005Fall_ExamII - Biol223_Smith Exam...

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Biol223_Smith Exam II 21 December 2004 PAGE 1 of 6 Exam # Biology 223 Fall 2004 Exam II, 21 December 2004 7:00-8:50 pm EXAM COPY # No notes, no calculators, no phones. Write your name and student ID number at the top of this page. Use non-erasable ink, write on the back if needed. Write legibly and succinctly, using proper terminology. Show your work and justify your conclusions, partial credit may awarded. Negative points may be assigned to unjustified, frivolous and grossly incorrect answers. Russell chapters 7, 8, 9, 10, 13, 14. 100 points possible, 110 minutes. Note points per question and spend your effort accordingly! codon chart Second base
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Biol223_Smith Exam II 21 December 2004 PAGE 2 of 6 Exam # Question 1 for 10 points Dr. Kurios receives a bacterial expression strain transformed with an over-expressing plasmid from a colleague. To check that he received the correct plasmid, he digests it with three enzymes, A, B, and C. The following fragments were observed in the respective single and multiple digests: size\digest A B C A+B A+C B+C A+B+C 4121 3128 5043 3128 4121 3128 3128 922 1915 985 745 1162 985 922 177 753 745 8 177 8 Construct map of the plasmid and present it as an ordered list of each site and the distance between it and the next (answer in the form X 121 Y144 Z 169 Z 196 Y 225 Z 256...). You may begin at any site in the plasmid, but list each site and fragment length only once. For a bonus: How many different maps are compatible with these data (not counting those that are circular permutations and reflections of how the map is presented)? Question 2 for 10 points Write out the sequence 5'-3'of all the DNA products to be found in the following two reactions, in which all reactions have the primer: 5'-TCACCACCCTGA-3' and the template:
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Biol223_Smith_08_2004-2005Fall_ExamII - Biol223_Smith Exam...

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