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Biol223_Smith_11_2004-2005Spring_ExamII -...

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Biol223_Smith_Exam_II 13 May 2005 PAGE 1 of 6 Exam # Biology 223 Spring 2005 Exam II, 13 May 2005, 6:00-7:50 pm EXAM COPY # No notes, no calculators, no phones. Write your name and student ID number at the top of this page. Use non-erasable ink, write on the back if needed. Write legibly and succinctly, using proper terminology. Show your work and justify your conclusions, partial credit may awarded. State any important assumptions and indicate ambiguity. Negative points may be assigned to unjustified, frivolous, and grossly incorrect answers. Russell chapters 8, 9, 10, 13, 14, 16. 100 points possible, 110 minutes. Note points per question and spend your effort accordingly! codon chart Second base
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Biol223_Smith_Exam_II 13 May 2005 PAGE 2 of 6 Exam # Question 1 for 15 points The diagram below represents a hypothetical human chromosomal region, with the locations of restriction sites indicated by vertical lines and location in kb (bp 1 is beginning of region). A probe spanning 2-5 kb is used in a Southern blot. The sites at 1 kb and 16 kb are found in everyone, but the human population contains individuals with every possible combination of restriction sites 3, 4, and 8 present or not. a) How many different combinations are possible? b) For each combination, what would be the size of fragments revealed by the probe in the Southern blot? c) What is the proper term to describe these differences? (spelled out for full credit) Question 2 for 10 points A section of a genome is mapped with probes to SNPs: A, B, C, D, E, F, and G. In reality, the probes are located in alphabetical order, with distances A-B = 1 kb, B-C = 0.2 kb, C- D = 5 kb, D-E = 1.2 kb, E-F = 0.01 kb, F-G = 1.5 kb. If the genomic DNA is randomly fragmented, and fragments in the size range 1.8 - 2.2 kb are isolated and
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