Biol223_Smith_17_2005-2006Spring_ExamII -...

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Biol223_Smith_2005-2006Spring_ExamII 12 May 2006 PAGE 1 of 5 Biology 223 Spring 2005-2006 Exam II, 12 May 2006 EXAM COPY # No notes, no calculators, no phones. Write your name and student ID number at the top of this page. I prefer ink. Write your answer where indicated, if possible, write on the back if needed. Write legibly and succinctly, using proper terminology. If I cannot read or understand your answer, you may receive no credit. You must show your work and justify your conclusions, partial credit may be awarded. State any important assumptions and indicate ambiguity. Negative points may be assigned to unjustified, frivolous, and grossly incorrect answers. 100 points possible Russell, 2nd edition, chapters 8, 9, 10, 11, 12, 15, 16, 18 Note points per question and spend your effort accordingly! codon chart Second base
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Biol223_Smith_2005-2006Spring_ExamII 12 May 2006 PAGE 2 of 5 Question 1 for 15 points A short double-stranded fragment of DNA is made by PCR, in which the upper strand is fluorescently-labeled at its 5’-end. Assuming complete digestion in every case, indicate the length (nt) of the fluorescent product of digestion with each of the restriction enzymes. 5’-*CCCGGGATGCGGCCGCTGCAGCATCCGAATTCCCGGG-3’
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This note was uploaded on 02/23/2010 for the course BIOL 223 taught by Professor Smithanddarwiche during the Spring '10 term at American University of Beirut.

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Biol223_Smith_17_2005-2006Spring_ExamII -...

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