Biol223_Smith_18_2005-2006Spring_Final

Biol223_Smith_18_2005-2006Spring_Final -...

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Biol223_Smith_2005-2006Spring_Final 07 June 2006 PAGE 1 of 9 Biology 223 Spring 2005-2006 Final, 07 June 2006 EXAM COPY # No notes, no calculators, no phones. Write your name and student ID number at the top of this page. I prefer ink, but pencil may allow you to finish more legibly. Write your answer where indicated, if possible, and if needed, write on the back. Write legibly and succinctly, using proper terminology. If I cannot read or understand your answer, you may receive no credit. You must show your work and justify your conclusions, partial credit may be awarded. State any important assumptions and indicate ambiguity. Negative points may be assigned to unjustified, frivolous, and grossly incorrect answers. 100 points possible per section Russell, 2nd edition Section I chapters 1-7 Section II chapters 8-12, 15, 16, 18 Section III chapters 19, 20, 22 Note points per question and spend your effort accordingly! codon chart Second base
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Biol223_Smith_2005-2006Spring_Final 07 June 2006 PAGE 2 of 9 Question 1 for 20 points a) identify each base as A, C, G, T, U, or as other (O) by labeling it such in the center of its 6-member ring. b) identify each base pair as having standard Watson-Crick Geometry (WC) or as being non-standard (NS) between the bases. c) for each standard base, circle the atom to which ribose or deoxyribose would be bonded in standard DNA or RNA. N N H NH N N H 2 O N NH NH 2 O N N NH N N H 2 NH NH O O N NH NH N NH 2 O N NH NH 2 O N N NH N N H 2 NH NH O O N N H NH N N H 2 O NH NH O O N N NH N NH 2 N NH N H 2 O N NH NH N NH 2 O NH N H O O N N NH N N H 2 NH NH O O N N NH N NH 2 N NH N H 2 O
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Biol223_Smith_2005-2006Spring_Final 07 June 2006 PAGE 3 of 9 Question 2 for 30 points In how many fragments would you expect each of the following restriction enzyme digests to cut a double- stranded, circular DNA of random (arbitrary) sequence, 10,000 bp in length? Only set up the calculations. S = G or C; W = A or T; M = A or T; K = G or T; D = A, G, or T; H = A, C, or T a) MboI 5’-GAT/C-3’ b) BsbI 5’-CAA/CAC-3’ c) BseSI 5’-GKGCM/C-3’ d) SduI 5’-GDGCH/C-3’ e) both BseSI and SduI together f) what proportion of SduI sites will produce overhangs compatible with BseI sites? Question 3 for 20 points In a prototrophic strain of Neurospora crassa , intermediate B is made from components of minimal medium by enzyme 1, et cetera. Consider the following hypothesis, where compounds E and I are essential, but compound H is toxic if not metabolized into I . Consider the phenotypes (growth or no growth) that might be expected to result from deletions of single genes 1-7, and how that phenotype may be suppressed by the addition of single
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Biol223_Smith_18_2005-2006Spring_Final -...

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