Biol223_Smith_21_2006-2007Fall_Final

Biol223_Smith_21_2006-2007Fall_Final -...

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Unformatted text preview: Biol223_Smith_2006-2007Fall_Final 22 January 2007 PAGE 1 of 8 Biology 223 Fall 2006-2007 FINAL, 22 January 2007 EXAM COPY # No notes, no calculators, no phones. Write your name and student ID number at the top of this page. I prefer ink. If possible, write your answer where indicated, other write on the back. Write legibly and succinctly, using proper terminology. If I cannot read or understand your answer, you may receive no credit. You must show your work and justify your conclusions, partial credit may be awarded. State any important assumptions and indicate ambiguity. Negative points may be assigned to unjustified, frivolous, and grossly incorrect answers. 100 points possible each section Section I Russell, 2nd edition, chapters 1-7 Section II chapters 8, 9, 10, 11, 12, 15, 16, 18 (lac operon) Section III chapters 18 (trp operon), 22 Note points per question and spend your effort accordingly! codon chart Second base Biol223_Smith_2006-2007Fall_Final 22 January 2007 PAGE 2 of 8 SECTION I Question 1 for 15 points a) Identify any normal base found in unmodified DNA or RNA (A, C, G, T, or U) b) for those normal bases, circle the atom by which it would be attached to the (deoxy)ribose, (the glycosidic bond atom) c) identify any base pair with Watson-Crick geometry (found in A- and B-form DNA and RNA) Question 2 for 20 points a) How many different mRNA sequences can code for the peptide amino-Glycine Serine-Asparigine-Leucine-Isoleucine- Arginine? b) How many contain no adjacent cytosines (CC)? Question 3 for 15 points Design an 18 nt DNA probe set suitable to detect an mRNA coding for the polypeptide sequence: amino-Met-Ala-Gln-Arg-Ser-Asp-Ile-Glu-Asn-Phe-Ala-Tyr-Gly-Cys-Pro-Asn-His-Leu-Trp-Val-Arg a) Choose the peptide fragment most appropriate for this purpose: b) How many individual oligos must be represented in the degenerate probe? c) Write the sequence 5’->3’as one oligonucleotide, indicating degeneracy with N=any base, R=purines, Y=pyrimidines. Question 4 for 20 points Random polymers of RNA were used to elucidate the genetic code. Using the codon chart, predict all amino acids that would be present in a protein if a random copolymer made from the monomers A and U were translated. N N H NH N N H 2 O N NH NH 2 O N N NH N N H 2 NH NH O O N NH NH N NH 2 O N NH NH 2 O N N NH N N H 2 NH NH O O N N H NH N N H 2 O NH NH O O N N NH N NH 2 N NH N H 2 O N NH NH N NH 2 O NH N H O O N N NH N N H 2 NH NH O O N N NH N NH 2 N NH N H 2 O Biol223_Smith_2006-2007Fall_Final 22 January 2007 PAGE 3 of 8 Question 5 for 20 points A double-stranded DNA fragment was isolated from a cDNA library, and is part of an open reading frame....
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This note was uploaded on 02/23/2010 for the course BIOL 223 taught by Professor Smithanddarwiche during the Spring '10 term at American University of Beirut.

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Biol223_Smith_21_2006-2007Fall_Final -...

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