Biol223_Smith_23_2007-2008Fall_FINAL -...

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Biol223_Smith_2007-2008Fall_FINAL 23 January 2008 PAGE 1 of 6 Biology 223 Fall 2007-2008 FINAL, 23 November 2008 No notes, no calculators, no phones, etc. Write your name and student ID number at the top of this page and most importantly , on the answer page . Only the answer page will be graded, you must place your answers in the space provided. Write legibly, if I cannot read or understand your answer, you will receive no credit. If it takes too much effort, you may lose points. Unanswered questions receive zero credit. Within parts of multi-part questions, incorrect answers will subtract from correct parts. Unjustifiable, frivolous, and grossly incorrect answers may be assigned negative points. Thus, it is possible to receive a negative score on the exam. If you find any mistakes of mine in the question, note it on your exam answer page and you may receive extra credit. 100 points possible each section. Part I: Russell, 2nd edition, chapters 1, 2, 3, 4, 11, 12, 15, 16 Part II: Russell, 2nd edition, chapters 18, 19 (Lac operon only) Note points per question and spend your effort accordingly!
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Biol223_Smith_2007-2008Fall_FINAL 23 January 2008 PAGE 2 of 6 SECTION I Question 1 for 10 points (2 minutes) Polio is a positive-stranded RNA virus that replicates via a negative-stranded RNA intermediate. Assuming the positive strand is 10,000 nt long and 20% A, 25% C, 30% G. a) About how many uridines would be in the negative strand (answer with a whole number)? b) About how many times would you expect the sequence 5’-AUG-3' to occur in the positive strand (answer as the nearest whole number)? Question 2 for 10 points (1 minute) The single strand of DNA below was annealed to two radioactively-labeled RNA primers. The radioactively labeled nucleotides are shown underlined. Calculate the total lengths of the radioactive, single-strand product(s) expected from the addition of Escherichia coli DNA polymerase I and only dATP, dCTP, and dGTP in suitable buffer (Mg++, etc). Your answer should be one or more whole numbers. G
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Biol223_Smith_23_2007-2008Fall_FINAL -...

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