Biol223_Smith_25_2007-2008Spring_FINAL -...

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Unformatted text preview: Biol223_Smith_2007-2008Spring_FINAL 5 June 2008 PAGE 1 of 6 Biology 223 Spring 2007-2008 FINAL, 5 June 2008 No notes, no calculators, no phones, etc. Write your name and student ID number at the top of this page and most importantly , on the answer page . Only the answer page will be graded, you must place your answers in the space provided. Write legibly, if I cannot read or understand your answer, you will receive no credit. If it takes too much effort, you may lose points. Unanswered questions receive zero credit. Within parts of multi-part questions, incorrect answers may subtract from correct parts. Unjustifiable, frivolous, and grossly incorrect answers may be assigned negative points. Thus, it is possible to receive a negative score on the exam. If you find any mistakes of mine in the question, note it on your exam answer page and you may receive extra credit. 100 points possible each section. Part I: Russell, 2nd edition, chapters 1, 2, 3, 4, 11, 12, 15, 16 Part II: Russell, 2nd edition, chapters 18 (fine mapping and complementation), 19 (Lac operon only) Note points per question and spend your effort accordingly! Biol223_Smith_2007-2008Spring_FINAL 5 June 2008 PAGE 2 of 6 SECTION I [100 pts] Question 1 for 16 points (5 minutes) Describe the 8 base pairs shown below in the table, writing Y (yes) or N (no) in the table to indicate whether it has Watson-Crick geometry (row WC), and the number (0, 1, or 2) of standard bases found in it (row #). pair 1-2 3-4 5-6 7-8 9-10 11-12 13-14 15-16 WC # Question 2 for 16 points (5 minute) The single strand of DNA below was annealed to two radioactively-labeled RNA primers: 5’- UG ACUUG CUG A- 3’ and 5’- GG UUCUAUAUACG- 3’. The RNAs are radioactively labeled only at G nucleotides (shown underlined). Calculate the total lengths of the radioactive, single-strand product(s) expected from the addition of Escherichia coli DNA polymerase I or III in suitable buffer (Mg++, etc), without exposure to RNase, and the following dNTPs (your answers should be one or more whole numbers in each box): 5-ATCCGTCCGTCAGCAAGTCATGGTTCTATATACGCTTCGTATATAGAACCCTGACTTGCTGAG-3 dNTPs added dATP dATP+dCTP dATP+dCTP+dGTP all dNTPs DNA pol I...
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This note was uploaded on 02/23/2010 for the course BIOL 223 taught by Professor Smithanddarwiche during the Spring '10 term at American University of Beirut.

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Biol223_Smith_25_2007-2008Spring_FINAL -...

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