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Unformatted text preview: BIOL223_Smith_2008-2009Fall Page 1 of 7 BIOL 223 Fall 2008-2009 EXAM I, 14 November 2008 -No notes, no calculators, no phones, etc.-Write your name and student ID number at the top of each page and most importantly , on the answer page . -Only the answer page will be graded, you must place your answers in the space provided. Keep the exam pages attached so I can check your work.-Write legibly, if I cannot read or understand your answer, you will receive no credit. If it takes too much effort, you may lose points.-Unanswered questions receive zero credit.-Within parts of multi-part questions, incorrect answers may subtract from correct parts. -Unjustifiable, frivolous, and grossly incorrect answers may be assigned negative points. Thus, it is possible to receive a negative score on the exam.-If you find any mistakes of mine in the question, note it on your exam answer page and you may receive extra credit. -100 points possible -Russell, 2nd edition, chapters 1, 2, 3, 4, 11, 12, 15, 16-Note points per question and spend your effort accordingly! BIOL223_Smith_2008-2009Fall Page 2 of 7 Question 1 for 16 points (5 minutes) Describe the 8 base pairs shown below in the table, writing Y (yes) or N (no) in the table to indicate whether it has Watson-Crick geometry (row WC), and the number (0, 1, or 2) of standard bases found in it (row #). pair 1 2 3 4 5 6 7 8 WC # Question 2 for 16 points (5 minutes) The single strand of DNA below was annealed to two radioactively-labeled RNA primers: 5’- UG ACCG CUG A- 3’ and 5’- GG UUCUAUAUACG- 3’. The RNAs are radioactively labeled only at G nucleotides (shown underlined). Calculate the total lengths of the radioactive, single-strand product(s) expected from the addition of Escherichia coli DNA polymerase I or III in suitable buffer (Mg++, etc), without exposure to RNase, and the dNTP(s) indicated (your answers should be one or more whole numbers in each box): 5’-ACTGCCAAGACTTCTTCAGCGGTCAGGTCATCTTCGTATATAGAACCGAACCTATGCCCAATGC-3’...
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