Biol223_Smith_26_2008-2009Fall_ExamI -...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: BIOL223_Smith_2008-2009Fall Page 1 of 7 BIOL 223 Fall 2008-2009 EXAM I, 14 November 2008 -No notes, no calculators, no phones, etc.-Write your name and student ID number at the top of each page and most importantly , on the answer page . -Only the answer page will be graded, you must place your answers in the space provided. Keep the exam pages attached so I can check your work.-Write legibly, if I cannot read or understand your answer, you will receive no credit. If it takes too much effort, you may lose points.-Unanswered questions receive zero credit.-Within parts of multi-part questions, incorrect answers may subtract from correct parts. -Unjustifiable, frivolous, and grossly incorrect answers may be assigned negative points. Thus, it is possible to receive a negative score on the exam.-If you find any mistakes of mine in the question, note it on your exam answer page and you may receive extra credit. -100 points possible -Russell, 2nd edition, chapters 1, 2, 3, 4, 11, 12, 15, 16-Note points per question and spend your effort accordingly! BIOL223_Smith_2008-2009Fall Page 2 of 7 Question 1 for 16 points (5 minutes) Describe the 8 base pairs shown below in the table, writing Y (yes) or N (no) in the table to indicate whether it has Watson-Crick geometry (row WC), and the number (0, 1, or 2) of standard bases found in it (row #). pair 1 2 3 4 5 6 7 8 WC # Question 2 for 16 points (5 minutes) The single strand of DNA below was annealed to two radioactively-labeled RNA primers: 5’- UG ACCG CUG A- 3’ and 5’- GG UUCUAUAUACG- 3’. The RNAs are radioactively labeled only at G nucleotides (shown underlined). Calculate the total lengths of the radioactive, single-strand product(s) expected from the addition of Escherichia coli DNA polymerase I or III in suitable buffer (Mg++, etc), without exposure to RNase, and the dNTP(s) indicated (your answers should be one or more whole numbers in each box): 5’-ACTGCCAAGACTTCTTCAGCGGTCAGGTCATCTTCGTATATAGAACCGAACCTATGCCCAATGC-3’...
View Full Document

Page1 / 7

Biol223_Smith_26_2008-2009Fall_ExamI -...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online