Biol223_Smith_27_2008-2009Fall_FINAL

Biol223_Smith_27_2008-2009Fall_FINAL - BIOL...

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BIOL 223_Smith_2008-2009Fall_FINAL Page 1 of 7 BIOL 223 Fall 2008-2009 Smith FINAL, 27 January 2009 Notes, calculators, phones, and communication with other students is forbidden. Write your name and student ID number at the top of this page and most importantly , on the answer page . You must show your work, even though only the answer page will be graded. You must place your answers in the space provided. Keep the exam pages attached so I can check your work. If you do not show your work, you may not receive credit. Write legibly, if I cannot read or understand your answer, you will receive no credit. If it takes too much effort, you may lose points. Unanswered questions receive zero credit, and within parts of multi-part questions, incorrect answers may subtract from correct parts. Unjustifiable, frivolous, and grossly incorrect answers may be assigned negative points. Thus, it is possible to receive a negative score on the exam. If you find any mistakes of mine in the question, note it on your exam answer page and you may receive extra credit. The exam has two sections, section I has the same coverage as Exam I, and section II has material presented since Exam I. Section I has 100 points possible. It covers Russell, 2nd edition, chapters 1, 2, 3, 4, 11, 12, 15, 16 Section II has 100 points possible. It covers parts of Russell’s chapters 18 and 19 Note points per question and spend your effort accordingly!
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BIOL 223_Smith_2008-2009Fall_FINAL Page 2 of 7 SECTION I Question 1 for 10 points (3 minutes) Consider a random sequence of single stranded RNA 100 000 nucleotides long, with an unbiased base composition (one quarter A, one quarter C, one quarter G, and one quarter U). N refers to any, Y refers to pyrimidine, R refers to purine. a) How many times would you expect the sequence 5’-CAUNY-3' to occur? b) How many times would you expect 5’-RYYNA-3' to occur in a 100 000 nt RNA made with only A, C, and G? Q1 a b Question 2 for 10 points (3 minutes) The single strand of DNA below was annealed to two RNA primers that are labeled at their 5’-ends: 5’-* AGACCGCUGA- 3’ and 5’-* GGUUCUAUAUACG- 3’. Calculate the total lengths of the radioactive, single- strand product(s) expected from the addition of Escherichia coli DNA polymerase I or III in suitable buffer (Mg++, etc), without exposure to RNase, and the dNTP(s) indicated (your answers should be one or more whole numbers in each box): 5’-ACTGCCAATCTTCT
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This note was uploaded on 02/23/2010 for the course BIOL 223 taught by Professor Smithanddarwiche during the Spring '10 term at American University of Beirut.

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Biol223_Smith_27_2008-2009Fall_FINAL - BIOL...

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