Oct 22 2009

Oct 22 2009 - BIEB 132 October 22, 2007 Ron Burton Lecture...

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I. Molecular tools in marine biology - why? A. Identifcation oF organisms - each species is unique 1. "tree oF liFe" based on DNA sequences 2. diversity within species useFul For paternity and ecological studies B. Identifcation and quantifcation oF DNA, mRNA and proteins reveals "activities" 1. DNA sequences demonstrate that a capability may exist 2. mRNA studies show when gene is turned on and oFF 3. Protein assays directly show Functional activity BIEB 132 Ron Burton October 22, 2007 Lecture 9: Molecular Tools in Marine Biology
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II. Methodology A. DNA amplifcation and sequencing B. RNA and Protein based techniques III. Applications A. Species identifcations - “DNA barcodes” 1. Matching larval Forms with adults 2. ±isheries Forensics (whales, abalone etc.) 3. Gut contents B. Population structure and natural history C. Microbes - bacteria, viruses, phytoplankton, symbionts (many can’t be cultured)
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Reading assignment: Burton, R.S. 2009. Molecular markers, natural history, and conservation of marine animals. BioScience (in press).
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Birch Aquarium: Hey Ron -- can you use that DNA mumbo-jumbo to clear up a species identification problem? Some seahorses we raise and send to other aquaria… Ron: I don’t know much about seahorses, (meanwhile I check: http://www. ncbi . nlm . nih .gov/ ) Some years back, I got a phone call… Hippocampus erectus Hippocampus kuda Example: seahorse species identifcation For the Birch Aquarium Using DNA as a species identifcation tool
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GenBank Taxonomy Browser…
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Sequences of a gene called cytochrome b are available for many seahorse species, so if I can sequence that gene, I can compare…
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This note was uploaded on 02/23/2010 for the course BIEB BIEB 132 taught by Professor Hastings during the Fall '09 term at UCSD.

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Oct 22 2009 - BIEB 132 October 22, 2007 Ron Burton Lecture...

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