04EnzymesParameterEstimation

04EnzymesParameterEstimation - 1 Wouldnt it be nice if ....

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2 Wouldn’t it be nice if …. One equation could explain: Enzyme reactions (10 nm) Cell growth (10 μm) Cell function like directed migration Tissue/organ function (10 mm) Reaction of huge commercial bioreactors (10 m) We will go over that today. Key equation, concept. Will be used throughout course in different variations.
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3 Engineers – Scientists BME – Bridges between Scientist …. What is the mechanism of catalysis? What are hypothesis I can think of? What are clever experiments to test this? Engineers …. How can I maximize the use of this enzyme? What factors affect reactions (heat, mixing, . .) How can I quantitatively model and optimize? Textbook page 3
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4 Enzymes Structure and Mechanism of Catalysis S P S Δ G Δ G o -d [S]/ dt = ν [S ] = [S]( kT/h )exp ( Δ S /R )exp (- Δ H /RT ) Textbook P. 58
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5 Engineers – Scientists BME – Bridges between Scientist …. What is the mechanism of catalysis? What are hypothesis I can think of? What are clever experiments to test this? Engineers …. How can I maximize the use of this enzyme? What factors affect reactions (heat, mixing, . .) How can I quantitatively model and optimize? Textbook page 3
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6 Single Substrate Michaelis- Menten Kinetics S ubstrate P roduct E nzyme [E] : Brackets means concentration of that thing
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7 1. Rate of product accumulation increases over time. (first phase greatly exaggerated; usually can’t see) 2. Product concentration increases linearly with time. 3. Substrate is depleted, so the curve starts to level off. How does product concentration change with time? Constant [E]; [S] at time 0 = S 0 ; [P] at time 0 = 0 V
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8 How does enzyme velocity change with [S]?
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04EnzymesParameterEstimation - 1 Wouldnt it be nice if ....

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