06OtherAspectsofSingleEnzymeReactions-InhibitionpHTemp

06OtherAspectsofSingleEnzymeReactions-InhibitionpHTemp -...

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1 Reversible Inhibition Competitive Uncompetitive Noncompetitive V max [S] K m (1 + ) + [S] v = [I ±] K I V max [S] K m (1 + ) + [S] v = [I ±] K I V max [S] K m + [S] (1 + ) v = [I ±] K I [S] (1 + )
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2 Competitive Inhibition E + S ES E + P + I EI k 1 k -1 k 2 K I V max [S] K m (1 + ) + [S] v = [ I ] K I or K i K i You expect TS analog inhibitors to be competitive inhibitors. So how would you tell?
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3 In uncompetitive inhibition , the inhibitor binds only to the enzyme-substrate complex. E + S ES E + P k 1 k -1 k 2 + I ESI K’ I K’ I = [ ES ] [ I ] [ ESI ] Uncompetitive Inhibition
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4 All lines have identical slopes and are parallel. Uncompetitive inhibitors alter both the K m and the V max . 0 2 4 6 8 10 12 14 16 18 -2 -1.5 -1 -0.5 0 0.5 1 1.5 2 2.5 3 [ I ] = 2 [ I ] = 1 [ I ] = 0 1 / velocity 1 / [ S ] Uncompetitive Inhibition
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5 In noncompetitive inhibition , the inhibitor binds to both the enzyme alone and to the enzyme-substrate complex. E + S
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This note was uploaded on 02/23/2010 for the course BME 321 taught by Professor Shuichitakayama during the Winter '10 term at University of Michigan.

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06OtherAspectsofSingleEnzymeReactions-InhibitionpHTemp -...

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