06zOtherAspectsofSingleEnzymeReactions-immobilization

06zOtherAspectsofSingleEnzymeReactions-immobilization - 25...

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Unformatted text preview: 25 26 27 Enzyme Immobilization 28 Enzyme Immobilization 29 Surface adsorption: van der Waals, dispersion forces Covalent binding Desorption common due to hydrodynamic forces cross-linking with glutaraldehyde mitigates this problem Entrapment in polymer beads Ca-alginate agar k-carrageenin polyacrylamide collagen solid matrices (activated-C, porous ceramic) Enzyme Immobilization 30 Enzyme Immobilization 31 32 33 34 35 Comparison of Immobilization Methods 36 Enzyme Immobilization 37 Enzyme Immobilization Diffusional limitations Depend on nature of support material Hydrodynamic conditions Distribution of enzyme on surface/inside of support material ] [ diffusion of rate maximum reaction of rate maximum max b L S k V Da = = Mass transfer coefficient (cm/s) Substrate conc. In bulk liquid (g/cm 3 ) 38 Enzyme Immobilization - Surface Da >>1 : Diffusion limiting At steady state, reaction rate equals mass transfer rate: ] [...
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06zOtherAspectsofSingleEnzymeReactions-immobilization - 25...

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