09HowCellsGrow-GrowthKinetics

09HowCellsGrow-GrowthKinetics - Single Cell Type; Batch...

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Single Cell Type; Batch Reactor S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number
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Batch Cell Growth Cell growth is an autocatalytic reaction substrates + cells products + more cells Rate of growth (mass) Net specific growth rate: Rate of growth (cell number) Net specific replication rate: Gross specific growth rate – Loss of cell mass
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How Cells Grow 12 3 4 5 - Log Scale, Exponential P. 161
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Phases of Batch Growth Cycle Lag Phase Adaptation of cells to new environment Nutrients, growth factors Age In Enzyme Reaction, initial lag phase was very short 1
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Phases of Batch Growth Cycle Exponential Phase Balanced growth Growth rate independent of nutrient concentration Enzyme Reaction Was linear 2 Why do we call this “exponential” phase?
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Balanced growth Doubling time of cell mass = Doubling time of cell number Growth rate depends only on amount of cells there are that can divide (not nutrient) Doubling Time
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Phases of Batch Growth Cycle Declining Growth Phase Nutrient depletion Unbalanced growth (Cell size composition changes) 3 Enzyme Reaction Was Similar
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Phases of Batch Growth Cycle Stationary Phase No growth or growth = death Production of secondary metabolites Endogenous metabolism (if substrate depleted) May result from accumulation of inhibitory compound(s) Enzyme Reaction Was Similar 4 What are two possible reasons to show stationary?
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If Substrate (S) is zero, μ g is zero (no growth) Even if cell number remains same Cell mass may decrease.
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09HowCellsGrow-GrowthKinetics - Single Cell Type; Batch...

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