10Oxygen-Bioreactors

10Oxygen-Bioreactors - Single Cell Type; Batch Reactor...

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Single Cell Type; Batch Reactor S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number
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Substrate Limited Cell Growth E. coli growth on glucose (glucose is limiting substrate) How would you model this growth kinetics?
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Comparison of conc. driving forces and uptake rates for glucose and oxygen by yeast Problems encountered in oxygen transport can be illustrated by comparing transport of glucose vs oxygen; 1% Sugar (glucose) Broth O 2 sat @ 25 o C Conc. in bulk broth 10,000 ppm approx. 7 ppm Critical conc 100 ppm 0.7 ppm Rate of demand 2.8 mmoles/g cells/h 7.7 mmoles/g cells/h Need to constantly supply!
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Different Measures of Oxygen ppm (parts per million) mg/L ……. . Pretty much similar to ppm mmole dm -3 ….. 1/32 of mg/L % (partial pressure) … Need Henry’s law ……. . 21% is air ………… ~ 7 mg/L at room temperature
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Critical dissolved oxygen levels for a range of microorganisms Organism TemperatureCritical dissolved o C Oxygen concentration (mmoles dm -3 ) Azotobacter sp. 30 0.018 E. coli 37 0.008 Saccharomyces sp. 30 0.004 Penicillium chrysogenum 24 0.022 Azotobacter vinelandii is a large, obligately aerobic soil bacterium which has one of the highest respiratory rates known among living organisms
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Tissue Oxygen Concentration Percentage (%) Ambient air 21 Artery 13 Vein 3.2 one marrow 5 Brain 3-4 Epidermis 3-5 Kidney 1.7-2.1 Liver 4.34 Muscle 2.7-4 Oviduct 5 Spleen 3.1 Testis 1.7 Air 21% Alveoli 14% Artery 13% Vein 3% Testis 1.7% Muscle 2.7-4% Oviduct 5% Bone Marrow 5% Brain 3-4% Mean Oxygen Concentration in Various Tissues Important for regulation Of function (growth, differentiation, death, Secretion, etc)
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RESPIRATION RATE (Demand) Oxygen Uptake Rate (OUR) = q O2 *X q O2 = mmoles of oxygen consumed per gram of dry weight (or mg O 2 /gm DCW hour) specific rate of oxygen consumption X = cell concentration (gm DCW/L) The effect of dissolved oxygen on the specific uptake rate (i.e respiration or growth) is described by Michaelis Menton or Monod type relationship Respiration rate ( q O2 ) = q O2 max . O 2 conc / ( Ks + O 2 conc) (mg O2/g DCW hour) or μ = μ max . C/ (Ks + C) where C = oxygen conc.
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Dissolved Oxygen Concentration q O2 C critical Effect of dissolved O 2 concentration on the q O2 of a microorganism Specific O 2 uptake increases with increase in dissolved O 2 levels to a certain point C crit
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Oxygen Requirement is Cell Dependent • Page 293 table.
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Oxygen transfer (Supply) k L
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This note was uploaded on 02/23/2010 for the course BME 321 taught by Professor Shuichitakayama during the Winter '10 term at University of Michigan.

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10Oxygen-Bioreactors - Single Cell Type; Batch Reactor...

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