PCR discussion - Jacob Zipperstein Post Lab Analysis...

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Jacob Zipperstein Post Lab Analysis Discussion : The results for our gel came out to be what we expected. We figured that at least one of our reactions would contain primer dimer. Primer dimer is when a small amplicon results from the extension of self-annealed primers. These reduce the available amount of primers for the PCR-reaction. Therefore the PCR affectivity is reduced because of this non-specific reaction. We only identified one primer dimer and that occurred in Jones reaction. In my reaction, no primer dimer could be identified. This means that the reagents all went to creating the desired product (amplifying a segment of the DNA) rather then creating a Primer dimer. However, my reaction wasn’t perfect. Faint bands were created surrounding the 500 bp band. This could possibly be due to overloading the gel, or adding to much DNA into the reaction. Our ladder came out to be what we expected. The enzyme cut along the DNA and produced specific base pair fragments. Our negative control also came out with expected results. No bands could be found in this
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This note was uploaded on 02/23/2010 for the course ENGLISH 1011 taught by Professor Thompson during the Spring '07 term at University of California, Berkeley.

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PCR discussion - Jacob Zipperstein Post Lab Analysis...

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