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Unformatted text preview: 216 H-W10- Dr. Masato Koreeda Date: January 11, 2010 Topic : ___Thin-Layer Chromatography __ page 1 of 2. Thin-Layer Chromatography (TLC) I. TLC- A highly sensitive analytical technique used to: (1) Determine the purity of a sample (# of components in a mixture) (2) Determine the identity of two compounds (3) Monitor the progress of a reaction •Requires only microgram or γ (10-6 grams!) quantities of a sample. •Every reaction to be performed in the lab should be monitored by TLC. II. TLC plates : glass plates coated with silica gel that is impregnated with a fluorescent dye (use a 254 nm UV lamp for detection of the spots). co-spot (i.e., A and B) solvent front A B d d A d travel distance of the solvent d A travel distance of compound A R f (ratio to front) value for A = d A / d • By definition, Rf values are between 0 and 1.0. • Best resolution is achieved in the Rf 0.3 - 0.7 range....
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