Alee's SI Worksheet for October 22 2009

Alee's SI Worksheet for October 22 2009 - BSC 2010 Alee's...

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BSC 2010 – Alee’s SI Review Sessions  Name: ________________________________ Thursday, October 22, 2009 and Friday, October 23, 2009 The Central Dogma of DNA DNA Replication Components of a replication fork Origin of replication:  Single on bacteria, multiple in Eukaryotes Leading Strand:  Continuous synthesis Lagging Strand:  discontinuous synthesis via Okazaki Fragments Okazaki Fragments:  Helicase:  Enzyme that untwists and separates the double helix at the replication  fork, thereby generating single strands Primase:  creates  RNA primer  needed for DNA synthesis; makes the 3’ hydroxyl  group available DNAP III:  major DNAP; synthesizes in the 5’ to 3’ direction DNAP I:  removes RNA primer and replaces with DNA DNA Ligase:  seals fragments of DNA together Parental DNA: Single-Stranded (SS) Binding Protein:  Binds to the now single-stranded DNA  to stabilize them while synthesis proceeds   Looking for extra help in Biology? Come to one of the SI review sessions! Times and more information posted on the Supplemental Instruction Webpage!
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BSC 2010 – Alee’s SI Review Sessions  Name: ________________________________ Thursday, October 22, 2009 and Friday, October 23, 2009 Picture of Replication Fork Video on Replication http://www.youtube.com/watch?v=teV62zrm2P0       http://www.youtube.com/watch?v=dIZpb93NYlw       (funny one  ) Rules and Directionality of Biochemistry 1. DNA synthesis cannot start from “scratch” but RNA synthesis can  a. Due to differences in RNAP and DNAP  2. DNAP and RNAP synthesize in the 5’ to 3’ direction Looking for extra help in Biology? Come to one of the SI review sessions! Times and more information posted on the Supplemental Instruction Webpage!
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BSC 2010 – Alee’s SI Review Sessions  Name: ________________________________ Thursday, October 22, 2009 and Friday, October 23, 2009 3. DNA polymerase adds nucleotides only to the 3' end (i.e., free 3’ OH) of growing DNA 
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This note was uploaded on 02/25/2010 for the course BSC 2010 taught by Professor Bowes during the Spring '08 term at University of Florida.

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Alee's SI Worksheet for October 22 2009 - BSC 2010 Alee's...

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