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Unformatted text preview: Metabolic Biochemistry BIBC 102 Winter 2007 Lecture 3, January 12, 2007 ENZYME KINETICS LNC Chapter 6 REACTANTS SUBSTRATES PRODUCTS k First order reactions: A P k Second order reactions: A + B P d[P]/dt = d[A]/dt = k [A] ln[A] = ln[A o ]  k t or [A] = [A o ] exp( k t) k First order reaction: A P d[P]/dt = d[A]/dt = d[B]/dt = k[A][B] = k[A] 2 1/[A] = 1/[Ao] + k t When [A] = [B] or when A + A products k Second order reaction: A + B P [A] time time time ln[A] 1/[A] [A] o ln[A] o 1/[A] o 1 st order 2 nd order TRANSITION STATE THEORY A C B D A C B D A C B D A catalyst serves in at least two ways: it binds and aligns the substrates it facilitates the redistribution of electrons (charges) it stabilizes the transition state Fig. 62 Fig. 63 k 1 k 2 E + S ES Products k1 CHAPTER 6 Enzymes and Enzyme Kinetics Derivation of MichaelisMenten equation • 1. equilibrium assumption: k1 >> k 2 • 2. steady state assumption: d[ES]/dt = 0 • Note: [E] = [E] total – [ES] Derivation of Michaelis Menten Equation from Wikipedia...
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This note was uploaded on 02/25/2010 for the course BIBC na taught by Professor Schfler during the Spring '10 term at UCSD.
 Spring '10
 Schfler

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