Lec 7 - formation of a Schiff base as a covalent...

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Lecture 7 January 24, 2007 Metabolic Biochemistry – BIBC102
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1) pyruvate + 2e - + 2H + lactate E’ o = - 0.185 volts 2) NAD + + 2e - + H + NADH E’ o = - 0.320 volts reversing reaction 3 also reverses the standard reduction potential 3) NADH NAD + + 2e - + H + E’ o = + 0.320 volts adding reactions 1 and 3: pyruvate + NADH + H + lactate + NAD + E’ o = + 0.135 volts 2200 G’ o = - n F E’ o = - 2 ( 96.5 kJ/V.mol) (+0.135 volts) = - 23.7 kJ/mol n is the number of electrons involved in the redox reaction
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Glu + ATP Glu 6-P + ADP a group transfer reaction
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Glu 6-P Fru 6-P an isomerization
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Fru 6-P + ATP Fru 1,6-bisphosphate a group transfer
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CH 2 0~P C O HOC H HC O H HC OH CH 2 O~P CH 2 0~P C O HOC H HC O H HC OH CH 2 O~P - + CH 2 0~P C O HOC H HC O HC OH CH 2 O~P H
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Breakage of a C – C bond facilitated by formation of a covalent intermediate ( protonated Schiff base ) with the enzyme
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Unformatted text preview: formation of a Schiff base as a covalent intermediate in the aldolase reaction DHAP GAP an isomerization Fru-1,6-bisP DHAP GAP a mixed anhydride a high energy phosphate a thioester intermediate a thioester intermediate 1,3-bisPG + ADP 3-PG + ATP LNC 15.7 (3rd edition) Hydrolysis ? LNC 15.8 (3rd edition) Substrate channeling 3-PG 2-PG an isomerization/mutation 2-PG PEP + H 2 O a dehydration reaction PEP + ADP PYR + ATP a group transfer reaction 1 Glucose 2 GAP 2 PYR 2 ATP 2 ADP 2 x 2ADP + 2 P i 2 x 2 ATP 2 ADP + 2 Pi 2 ATP 2 NAD+ 2 NADH + 2H+ LNC 14.3 LNC 14.13 LNC 14.3a LNC 14.3b LNC 14.3c Metabolic flux and its control LNC 15.16 (3rd ed.) LNC 15.12 End of Lecture 7 January 24, 2007 LNC 3rd ed....
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This note was uploaded on 02/25/2010 for the course BIBC na taught by Professor Schfler during the Spring '10 term at UCSD.

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Lec 7 - formation of a Schiff base as a covalent...

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