Class 20 - Molecular Variation: How to Assess it...

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Unformatted text preview: Molecular Variation: How to Assess it Mutagenesis and its affects Mutations arise spontaneously as well as by induction from the environment Mutations can be thought of as random events occurring over time They affect all aspects of information flow from DNA to phenotypes point mutants frame shift promoter splicing termination DNA replication 2 Causes of Mutations : Effects of different point mutations AMES TEST In this test mutagens are mixed with bacteria that have a single mutation that prevents growth in histidine- minus growth medium. If mutagen is present, the his- mutation will revert by back- mutation to wild type and allow growth without histidine. Ames Test Determine if substance is mutagenic Ames bacteria : Salmonella his- auxotroph Assay for conversion of his- auxotroph to prototroph in presence of potential mutagen 1. growth of colonies on minimal plates minus his 2. growth should be above spontaneous reversion rate to his+ No mutagen Mutagen Variation is a hallmark of living things Mutation rate is between 10-5 and 10-9 mutants per base per generation Mutation frequency is the prevalence of a particular mutation (i.e. CF) in a population...
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Class 20 - Molecular Variation: How to Assess it...

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