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Class 22 - at the telomeres(ends of human chromosomes...

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Markers and cytology James Watson (1928- ) Francis Crick (1916 – 2004) Nobel Laureates who changed the world of genetics
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Exam Monday 6:30-8:30PM Place: Right here, A2B70 MCDB Bring ID, #2 Pencil, calculator You need to know your student #. Friday will be a review class - please email to [email protected] questions you would like answered Additional review 3-5PM Monday Oct 20 in MCDB A120 2
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Karyotype analysis FISH = Fluorescence In Situ Hybridization Concepts of in situ hybridization Chromosome Painting • SKY 3
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Prepare metaphase cells (in mitosis how many chromatids corresponding to chromosome 6 are there?) Denatured DNA sticks to plate Fluorescently labeled single strands are hybridized to specific genes or sequences
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6 Use fluorescent dye substituted nucleotides to label DNA Hybridize to metaphase cells and look for signals
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Probe specific to sequences
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Unformatted text preview: at the telomeres (ends) of human chromosomes Probes for 6 different genes on chromosome 12 Chromosome painting A mixture of probes for genes known to map on only one chromosome 8 Spectral Karyotyping (SKY) Easy to see rearrangments 9 With FISH diagnoses are simpler Loss of an oncogene in a cancer cell (top two, loss of one of the two orange (abl) genes Sex typing - Y chromosome is red, X is green - blue is autosomal marker 10 Trisomy 21 - Downs Syndrome One of the only viable trisomies is easily diagnosed with FISH 11 With FISH diagnoses are simpler! Cell probed, at metaphase (why?) with a chromosome 21 painting mixture of probes 13 Translocation is easy to diagnose with chromosomal painting 14 S pectral K arY otype (SKY) provides a “bar code” to find smaller rearrangements 15...
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