Class 28 - Genome Arrangements and Rearrangements Pictured...

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Genome Arrangements and Rearrangements Pictured are Queen Victoria and Prince Albert of Great Britain with some of their descendants.
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2 What is inside a chromsome? DNA! Some functions of DNA Encoding proteins Control of gene expression Self-replication Facilitating recombination Permitting evolution Folding – maintaining stable karyotype Species isolation No function? Junk?
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3 DNA – the more the better? (not. ...) C-value = DNA Content per cell Organism DNA (pg) Organism DNA (pg) Grass 3.5pg Yeast 0.05pg Garden pea 5 pg Fruitfly 0.15pg Onion 16.8 pg Chicken 1.3pg Tulip 31 pg Human 3.2 pg E. coli 0.005pg Amphibian 0.6- 80.0pg Fish 5-10pg
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3 DNA – the more the better? The C-Value Paradox Organism DNA (pg) Organism DNA (pg) Grass 3.5pg Yeast 0.05pg Garden pea 5 pg Fruitfly 0.15pg Onion 16.8 pg Chicken 1.3pg Tulip 31 pg Human 3.2 pg E. coli 0.005pg Amphibian 0.6- 80.0pg Fish 5-10pg
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Distribution is approx. random and in mammals is 20-40,000bps with stem size of 200-300 or 1300 in drosophila. First seen by electron microscopy – confirmed by DNA sequencing Components of the genome Inverted Repeats
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Components of the genome Satellite DNA Defined by unusual “density” during centrifugation Called satellite because forms smaller peaks to the side of the bulk of DNA Shown to contain repeats of simple sequences of a few 10s of base pairs, represented more than 10 7 (25% of total genome!)
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Components of the genome What is satellite DNA? Each organism is different – found using in situ hybridization to be centromeric D. virillus Genome Fraction AC AAAC T 25% AT AAAC T 8% AC AAAT T 8%
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Components of the genome What is it for? DNA appears inert - no genes/no transcription No coding capacity Functions in recognition of centromeres by segregation apparatus (spindle microtubules) May function as an evolutionary "buffer" to insure segregation in the face of mutation In telomeres, involved in maintaining ends of chromsomes
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This note was uploaded on 02/25/2010 for the course MCDB 2150 taught by Professor Winey,mark during the Spring '06 term at Colorado.

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Class 28 - Genome Arrangements and Rearrangements Pictured...

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