Class 29 - Recombinant DNA Technology

Class 29 - Recombinant DNA Technology - It is half way...

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It is half way through the semester and we want to know how things are going Please take this survey: http://www.colorado.edu/sei/surveys/Fall08/fa08-mcdb2150-mids It is worth 4 Participation Points!!!! Due November 5th at 11 pm Your answers cannot affect your grade. Once you complete the survey, you will be given an anonymous 10-digit number. Ken will not be able to view your answers in a way that has your name or any identification number (for example your student ID) attached to it. Ken does not have access to the server where the survey results are stored.
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MCDB 2150 – Principles of Genetics +
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What is cloning? Organism cloning Clone (verb) – to propagate asexually from an individual. CC, Carbon Copy, was cloned from a female calico cat named Rainbow. CC and surrogate mom, Allie Genetic mom, Rainbow Gene cloning Clone (verb) – to isolate a gene and make many copies of it. Insulin was the first human gene cloned , and now the insulin protein can be generated in bacteria.
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Why clone a gene? Study the gene in vivo (in an organism) make antibodies knock down or delete gene over express gene introduce specific mutations into a gene ‘tag’ a specific protein Study individual proteins or protein complexes in vitro interaction studies enzymatic activity assays mRNA 5’ cap Produce a lot of a specific protein medical uses (insulin) scientific uses (restriction enzymes)
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Making Recombinant YFP (Your Favorite Protein) Recombinant DNA technology – isolating and manipulating DNA. Combining DNA from two different sources. 1. Digest gene of interest (YFG) and cloning vector with the same restriction enzymes 2. Mix and bind together the foreign and plasmid DNA 3. Introduce the plasmid into bacterial cells 4. Select for transformed cells 5. Produce recombinant protein – and experiment!
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1. Digest gene of interest (YFG) and cloning vector with the same restriction enzymes 2. Mix and bind together the foreign and plasmid DNA 3. Introduce the plasmid into bacterial cells 4. Select for transformed cells 5. Produce recombinant protein – and experiment! Making a recombinant protein Recombinant DNA technology – isolating and manipulating DNA. Combining DNA from two different sources.
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Cloning Vector or Plasmid A carrier for the cloned DNA segment.
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This note was uploaded on 02/25/2010 for the course MCDB 2150 taught by Professor Winey,mark during the Spring '06 term at Colorado.

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Class 29 - Recombinant DNA Technology - It is half way...

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