Class 33 - Gene mapping in humans 1 Exam Monday 6:30-8:30PM...

Info iconThis preview shows pages 1–13. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Gene mapping in humans 1 Exam Monday 6:30-8:30PM Place: Right here, A2B70 MCDB Bring ID, #2 Pencil, calculator You need to know your student #. Friday will be a review class - please email to krauter@colorado.edu questions you would like answered Additional review 3-5PM Monday Oct 20 in MCDB A120 2 Human gene mapping Genetic mapping What is a map? A conceptual representation of space Based on identified landmarks Has units of distance and sometimes direction between landmarks Two types of human maps Genetic Physical 3 Human gene mapping Physical Maps A physical map is based entirely on the DNA sequence The actual sequence is the ultimate map A set of ordered clones is a physical map A set of ordered FISH probes is a map A set of ordered RFLPs is a map 4 Human gene mapping Clone Maps A clone map is represented by a series of overlapping clones of DNA Depends on ability to identify clones containing overlapping DNA sequences Complicated by repetitive DNA 5 DNA to clone map PCR products Clone 1 Clone 2 Clone 3 A CONTIGuous set of overlapping clones is called a CONTIG Clone libraries contain subfragments of larger DNAs TERM: Library A collection of clones that contains fragments of DNA representing another organism Usually produced by random cloning of fragments of another genome Genomic library produced from nuclear DNA cDNA library produced from cytoplasmic mRNA Representation is the coverage of the cloned target DNA in the library 6 Human gene mapping Creating a contig from a library 7 Human gene mapping Creating a contig - step 1 This collection of clones is would be a representative library if all fragments 1-27 were part of it Human gene mapping Creating a contig - step 2 9 Human gene mapping Creating a contig - step 3 10 Recall that a commonly used probe is a unique PCR product specific for one and only one sequence in the genome. Such a probe is called a sequence tag site or STS Human gene mapping Final product 11 12 Please answer this question without talking to your neighbor Please dont whisper or share answers for this question, we promise you will get a chance to talk to your neighbor!! 13...
View Full Document

This note was uploaded on 02/25/2010 for the course MCDB 2150 taught by Professor Winey,mark during the Spring '06 term at Colorado.

Page1 / 41

Class 33 - Gene mapping in humans 1 Exam Monday 6:30-8:30PM...

This preview shows document pages 1 - 13. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online