Class 34 - Exam 3 review 1 Exam Monday 6:30-8:30PM Place:...

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Exam 3 review 1
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Exam Monday 6:30-8:30PM Place: Right here, A2B70 MCDB Bring ID, #2 Pencil, calculator You need to know your student #. Friday will be a review class - please email to krauter@colorado.edu questions you would like answered Additional review 3-5PM Monday Oct 20 in MCDB A120 2
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Repeats fall into two classes Tandem (i.e. rDNA, tDNA, Satellite DNA) and Interspersed (SINES, LINES) Rerun. ... 3
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Components of the genome Classes of Interspersed Repeats SINES LINES Retroviruses Multigene Families Pseudogenes 4
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Essentials Transposition can be mediated by enzymes contained in the “transposon” Insertion at the new site is generally not sequence specific so can interrupt genes
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Essentials Transposition can be mediated by enzymes contained in the “transposon” Insertion at the new site is generally not sequence specific so can interrupt genes
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Essentials Transposition can be mediated by enzymes contained in the “transposon” Insertion at the new site is generally not sequence specific so can interrupt genes TE
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Retrotransposition Gene is transcribed normally (spliced too!) Acted on by reverse transcriptase DNA copy is then reinserted into the chromosome “randomly”
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Retrotransposition Gene is transcribed normally (spliced too!) Acted on by reverse transcriptase DNA copy is then reinserted into the chromosome “randomly”
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Technical sidebar - blotting
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Technical sidebar - blotting
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Technical sidebar - blotting
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Technical sidebar - blotting
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Technical sidebar - blotting
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Technical sidebar - blotting
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Components of the genome How to prove transposition occured? Find the DONOR L1 gene for a transposition Problem - there are 10 5 copies L1 LINE - most only fragments of entire 4kb element Each L1 differs by 5% from consensus gene (average seq) Partial L1 element Structure of patients hemophilia gene DNA This L1 sequence differed from EVERY sequence in the child’s genome that was tested! AUG
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Components of the genome How to prove transposition occured? Find the DONOR L1 gene for a transposition Problem - there are 10 5 copies L1 LINE - most only fragments of entire 4kb element Each L1 differs by 5% from consensus gene (average seq) Partial L1 element Structure of patients hemophilia gene DNA This L1 sequence differed from EVERY sequence in the child’s genome that was tested! AUG
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A form of X-linked recessive muscular dystrophy can be caused by the deletion of a transposon-like element from the DMD gene. Below is a pedigree of a family with a member who has X-linked recessive muscular dystrophy. You use PCR to amplify the DMD gene from each member of this family and run the product of the reaction on a gel.
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This note was uploaded on 02/25/2010 for the course MCDB 2150 taught by Professor Winey,mark during the Spring '06 term at Colorado.

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Class 34 - Exam 3 review 1 Exam Monday 6:30-8:30PM Place:...

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